A comparative genetic diversity study among elite Bt and Non-Bt cotton varieties of Andhra Pradesh by RAPD markers
نویسنده
چکیده
The present work dealt with RAPD markers for genetic diversity comparison estimation in twenty three cotton varieties of Bt and non-Bt cotton cultivars belonging to two cultivated species of cotton in Andhra Pradesh state, India.Variations among twenty three different cotton varieties were analyzed with selected 40 RAPD primers using the polymerase chain reaction (PCR). All the primers produced polymorphic amplicons; however the extent of polymorphism varied with each primer in twenty three cotton varieties. Out of a total of 409 RAPD bands generated from the twenty three varieties, 257 bands perceived polymorphism. The maximum similarity of 94.6% was observed between Gossypium hirsutum and Bt cotton varieties and minimum similarity of 4.4% was observed between Gossypium arboretum and Bt cotton varieties respectively. Cluster analysis by the unweighted pair group method of arithmetic means (UPGMA) resulted in two broad clusters representing twenty three varieties of cotton in each. All the varieties could be discriminated from one another using combined profiles for 40 primers and the relationship among the Bt and non-Bt cotton varieties under study is presented in the dendrogram.
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